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Although the primers for this MLVA are designed for use with S. aureus only and cannot be used for other species the method itself is flexible and can be used for any bacterial species that carries VNTR loci.
To test whether the human-data trained GExposer works well on different species, the method was applied to plant (maize) RNA-seq data.
Receiving as input a list of one2one orthologous genes found in the genomes of two species, the method builds a set of reliable and non-overlapping synteny blocks and refines the regions that are not contained into them.
For T. vivax, a large and very mobile species, the method has been reported to detect 80percentt of cases where the numbers of trypanosomes per ml of blood are between 300 and 700, and 100percentt for numbers of more than 700 per ml.
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While this outcome may not hold for other locations or species, the methods we used can be implemented to help determine appropriate methodologies for particular research questions.
In recent years the pure-line method as outlined above has decreased in importance in the breeding of major cultivated species; however, the method is still widely used with the less important species that have not yet been heavily selected.
These were sub-cultured to obtain pure isolates of Bacillus species using the method of Aslim et al. [10].
You correctly write that Japan will limit whaling to its coastal waters, but fail to note that Japan will set catch limits at a level that does not negatively affect the sustainability of target species, using the method established by the International Whaling Commission.
As for other MLST methods, the user must know the species for the method to use the correct MLST scheme.
Based on the alignments, we identified PHAS loci from the 23 species using the method described in Xia et al. [ 23] (see Methods for details).
Consequently, this monophyletic cluster recognized as a putative species by the method of Pons et al. [ 28] could be without ambiguity named as Leopoldamys neilli.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com