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To investigate the recent demographic history of this species a minimum spanning network was created in Arlequin, version 3.01 [43] and drawn by hand.
For each species, a minimum of 50 colonies were sequenced for family-A and family C analysis.
After identification of recombinant clones, for diploid species, a minimum of two clones were sequenced, for tetraploid species, at least six clones were picked and sequenced.
For each species, a minimum of 12 different colonies was collected and used as replicates in the experiments, with the exception of F. selysi, for which only 10 colonies were obtained.
Thus, to make an unbiased assessment of the effect of network structure on avian carotenoid diversification, we built two kinds of networks for each species – a "minimum" and "maximum" networks.
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We also set a relatively high penalty value for losing a species, so that every solution represented all species with a minimum number of ecoregions.
Further, serotyping performed at a state public health laboratory to define the Salmonella species requires a minimum of 2 days, and for biphasic Salmonella species, such as S. Newport a minimum of 4 days (9 ).
We selected species with a minimum occurrence of 10 sites, which accounted for 48.1% of all species.
For species previously considered threatened or endangered, section 4(g)(1) of the Endangered Species Act requires the U.S. Fish and Wildlife Service in cooperation with the States to monitor a species for a minimum of five years after being removed from the List of Endangered and Threatened Wildlife and Plants to ensure they maintain non-threatened status.
Grasshopper species number varied between 21 and 1 per test area, and butterflies showed a maximum of 15 species and a minimum of none.
By setting the rate of reaction of equation 17 equal to k and the probability of reaction of equations containing any temporary species on the reactant side equal to 1, we reduce the artifacts due to the creation of the "temporary" species to a minimum.
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