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These results point out the specific chemical behavior of individual metals in estuarine sediments, and the need for direct confirmation of metal speciation in order to constrain predictive models that realistically assess the fate of metals in urban harbors and coastal sediments.
As in all other such studies, we assigned an equal cost for gene fusion, fission, duplication, loss, or speciation in order to avoid prior bias stemming from uncertainties relating to the relative frequency of these events [ 26] and their dependence on the particular genomes investigated [ 22, 23].
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In systems such as the EABH, which contain a high concentration of old endemics with limited and fragmented distributions in isolated highlands [ 34], a community approach may be necessary to clarify the prevalence of these speciation mechanisms in order to more fully understand the formation of its rich biota.
Therefore, here we will use the cDNA-AFLP technique and qPCR to compare the expression profiles between two ecotypes of Littorina saxatilis involved in an incomplete sympatric ecological speciation process [ 43- 45], in order to provide insights into the role of differential gene expression on ecological adaptation.
Speciation studies are thus required in order to evaluate the risk of the nAg, and to distinguish it from that of the released free Ag and silver complexes.
In order to understand speciation and its role in evolution, it is useful to know how much genetic change takes place during the course of species development.
The pH potentiometric data were elaborated in order to obtain appropriate speciation models and to determine the stability constants of complexes.
The results discussed above suggest that ecological specialisation and sexual selection have to work hand in hand in order to achieve adaptive speciation.
An apparent lack of morphological diversity could be attributable to non-adaptive speciation, but morphometric work would be required in order to quantify phenotypic diversity.
Representative strains of all neighbour species (the Harzianum-Catoptron Clade; [ 17, 18]) were included in order to determine the speciation history within the clade, to set up genetic borders for morphological H. lixii and T. harzianum species, and to be used as a negative control for recombination analyses.
In order to calculate speciation rates and assess speciation modes, a robust and detailed phylogenetic framework is required in order to determine evolutionary relationships and timing of cladogenetic events [41] [42].
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