The facility operated on a 12 h light/dark schedule (lights on at 0700 h) and animals had access to standard mouse food (Feeder and Grower diet, Specialty Feeds, WA) and water ad libitum.
Mice were fed ad libitum on NIH-31 diet (control) or methyl-donor supplemented NIH-31 (plus (per kg) 15 g choline, 15 g betaine, 7.5 g L-methionine, 150 mg zinc, 15 mg folic acid, 1.5 mg vitamin B12) (Specialty Feeds, Glen Forrest, WA, Australia) [17], [26].
Following the forced swim test, mice were food deprived for 24 h before being placed in an opaque white acrylic box (30×30×30 cm) that contained a food pellet chip (Feeder and Grower diet, Specialty Feeds, WA) placed on a 5×5 cm piece of filter paper.
All mice received the same fixed formulation diet (Specialty Feeds, Glen Forrest, Western Australia, Australia).
Standard rat pellet was purchased from Specialty Feeds (Glen Forrest, WA, USA), while palm oil was supplied by Yee Lee Edible Oils Sdn.
Mice had ad libitum access to normal salt diet (0.26% (w/ w) NaCl; AIN93M, Specialty Feeds, Australia) and water.
Standard rat pellet was from Specialty feeds (Glen Forrest, WA, USA), while palm oil was supplied by Yee Lee Edible oils Sdn.
Mice had free access to water and a pellet diet (Rat and mouse cubes; Specialty Feeds, Glen Forrest WA 6071, Australia).
Mice (6 weeks old) were given a diet containing 320 p.p.m. sulindac (Sigma-Aldrich; Specialty Feeds, Australia) for 1 week or control feed ad libitum.
After 20 weeks (140 days), the HFD fed mice were swapped onto a chow diet (4.8% of fat, SF04-001, Specialty Feeds, Western Australia, Australia) for 4 weeks.
These mice were allowed 2 weeks recovery postsurgery and, following baseline recordings, mice were spilt onto either chow (4.8% of fat, mouse and rat rodent chow diet, Specialty Feeds, Glen Forrest, Australia) or HFD (43% of fat, SF04-001, Specialty Feeds, Western Australia, Australia) for 20 weeks (140 days) in which recordings were taken every 13 to 15 days.
