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The search for HS1.2 polymorphisms was refined using the specialized alignment tool Trace-Archive BLAST http://blast.ncbi.nlm.nih.gov/Blast.cgi BLAST_SPEC=TraceArchive&BLAST_PROGRAMS=megaBlast&PAGE_TYPE=BlastSearch.nih.gov/Blast.cgi BLAST_SPEC=TraceArchive&BLAST_PROGRAMS=megaBlast&PAGE_TYPE=BlastSearch
Whole-genome alignment must allow for reads that span splice junctions; specialized alignment algorithms have been developed to address this problem (Li and Durbin 2009; Trapnell et al. 2009, 2010; Wu and Nacu 2010).
Most alignment software considers the primary sequence alone; some specialized alignment software can also include Watson Crick base pairs, but none adequately addresses the needs introduced by the rapid influx of both sequence and structural data.
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These specialized 16S rRNA alignment tools all incorporate information about the 16S rRNA secondary structure.
SeaView (Galtier et al., 1996) is a specialized cross-platform alignment editor developed for molecular phylogeny studies.
Second, the data exists in a specialized format, the Multiple Alignment Format (MAF; Supplementary Fig. S1).
However, these sophisticated visualization tools are specialized in handling intraspecies alignment results (i.e. query and reference are same species).
Alternatively, one might want to distinguish the probabilities that a base is paired upstream, downstream, or remains unpaired (4) Distance measures derived from these three quantities have been used in particular in specialized structure-aware sequence alignment algorithms for structured RNAs [Bonhoeffer et al., 1993; Dalli et al., 2006; Kruspe and Stadler, 2007].
Intracellular alignments of specialized organelles called magnetosomes are responsible for this behaviour.
There are also several specialized tools such as the alignment tool.
For transcriptome analysis, the ESTs are additionally aligned to the genome sequence of the organism using specialized programs like BLAT (BLAST like alignment tool) [ 18] to assist genome mapping and gene discovery.
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