Sentence examples for spe I from inspiring English sources

The DNA fragments encoding GmDof4 were obtained by digesting the pEB-GmDof4 with Spe I and Not I, and the fragments were then inserted into the site between Spe I and Not I in the plastid pCK at the downstream of the ubi promoter, resulting in vector pGreen0029-Ubi-GmDof4-Nos (pGmDof4).

The PCR product was digested with Spe I, and the resulting fragment was ligated to plasmid pVK51 prepared by digestion with Sca I (blunt-end) and Spe I.

Amplified zeocin products were digested with Spe I and subcloned into the Spe I site of the plasmid pMD19T-CBS, giving the plasmid pMD19T-C-Z (Figure2B).

Spe I digestion of the genomic DNA resulted in a unique hybridizing band but larger than 1.5-Kb.

The two additional sequences GGACTAGT and CCCAAGCTT in each primer were specially added for restriction enzyme Spe I and Hind III, respectively.

For construction of the overexpression cassette, the coding region of GLR1 was amplified and liagated to the 1460 vector by BamH I and Spe I.

The PCR fragment was digested with Spe I and Hind III restriction enzyme and then inserted into the Spe I-Hind III sites of the pMIR-REPORT plasmid (Ambion, USA).

The Spe I-digested PCR product was cloned into the Spe I site of the vector pSP64T-GR (Tada et al., 1997 ) to fuse Eomes in frame to the C-terminal GR fragment.

To construct pAeG, the Spe I- EcoR I fragment of the CAG promoter from pCAGGS [ 5] was inserted into pCeG.

The insert was then excised out by Spe I-Eco RV or Kpn I-Xho I digestion.

First, the Spe I- Xba I fragment (Z12) derived from pMDZ12 was ligated into pSK-Z3M plasmid which was digested with the same enzymes.