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SPC-A-1 (SPC) and LTEP-A-2 (LTE) were obtained from the cell bank of Chinese Academy of Science.

However, the expression levels of E-cadherin have no significant difference between SPC-A-1sci SPC-A-1sci SPC-A-1sciquanditative PCR analysis (Fig. 2C).

Total RNA of cultured SPC-A-1sci and SPC-A-1 cells were isolated using Trizol reagents (Invitrogen) according to the manufacturer's instructions.

These findings suggested that fibronectin play a crucial role in promoting cytoskeleton reorganization and morphological transition from SPC-A-1 to SPC-A-1sci cells.

As shown in Fig. 2A, distinct morphological differences between the SPC-A-1sci cell line and its parental SPC-A-1 cell line were observed in cell culture.

First, we employed wound healing assays to determine migratory abilities of SPC-A-1sci and SPC-A-1 cells on fibronectin coated or uncoated plates.

These results suggested that SPC-A-1sci cells acquire the increased abilities to migrate and invade, which further demonstrated that SPC-A-1sci cells have undergone EMT.

Of note, there is no significant difference in the mRNA level of E-cadherin between SPC-A-1sci and SPC-A-1 cells.

Compared with its parent cell line SPC-A-1, SPC-A-1sci was more aggressive in vitro, including increased potentials for cell spreading, migration and invasion.

Lenti-virus generation and infection of SPC-A-1sci cells were performed as described above.

We therefore examined the spreading ability of SPC-A-1sci and SPC-A-1 cells after plating on fibronectin coated or uncoated plates and found that SPC-A-1sci cells spread significantly faster than SPC-A-1 cells did on both conditions (Fig. 4A).