Sentence examples for spb in from inspiring English sources

To visualize the SPB pore (the protein structure that surrounds the core SPB and anchors the SPB in the NE), we exploited the fact that SPB diameter, and thus pore diameter, increases with ploidy (Byers and Goetsch, 1974; Bullitt et al., 1997).

We observed Nud1-YFP at the SPB in the majority of SPBs at later time points (83% at 60 min, 48/58), as illustrated in Figure 5C (60 min both cells have two Spc42-mTurquoise2 foci, and the cell with the larger bud that has progressed further in the cell cycle contains Nud1-YFP at both poles.

We observed that Kar5-TM3-GFP still enriched at the SPB in mitotic cells, demonstrating that the SPB-enriching factor is not restricted to pheromone treated cells.

We accordingly examined whether Pcp1 is involved in Cut12 recruitment to the SPB, but again Cut12 GFP localised normally to the SPB in pcp1-18 cells at 36°C.

Therefore, it is presumed that Ndc1 and Nbp1 also anchor the SPB in the NE through unknown binding interactions with the non-membrane core SPB.

Given the role of Cut11 in SPB insertion, we investigated whether pcp1-18 cells fail to reorganise the NE and the SPB in mitosis.

Based on the fact that Mps2 and Ndc1 are integral membrane proteins involved in SPB insertion into the NE (Chial et al., 1998; Munoz-Centeno et al., 1999), the donut shaped rings are likely SPBs in transverse section with Mps2 and Ndc1 localizing to the pore membrane around the SPB.

Large SPBs in ubc5Δ cells make up 13.6% ±1.0%of the population, which is similar to the percentage found in wild type SPB populations.

A comparable increase in the percentage of large SPBs in ubc4Δ cells was seen when SPB core component Spc42 was tagged with GFP (data not shown).

The quantification of background intensity, fluorescence intensity of Spc42-GFP and immuno-stained Tub4 was performed on planes having SPBs in focus.

The Spc110-mCherry signals at the SPBs in tub4-S74E, tub4-S100E and tub4-S360D cells were similar to TUB4 in WT cells (Figure 8D), suggesting that phosphorylation of Tub4 has no impact on Spc110 incorporation into SPBs.