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Combine the organic phases, transfer them into a new 2-ml PP tube and dry them by adding a spatula tip of anhydrous Na2SO4.
A linear scratch was made in each confluent culture with a 0.6 cm spatula tip and the cultures were returned to 37°C for 6 h.
To avoid acid traces, a spatula tip of CaCO3 was added before extraction with 1 mL of 100%% high-performance liquid chromatography (HPLC) grade acetone at 4 °C under a PPFD of 10 μmol m−2 s−1.
The product (2 g) was dissolved in 50 mL of MeOH/H2O (20:1) under sonication to dissolve the product and, after the addition of a spatula tip of 5% Pd/C, hydrogenated at room temperature and atmospheric pressure.
β-Globin could be amplified in only 89% of samples, due probably to the inhibitory effect of the wooden spatula tip, which remained in the sample at room temperature for several weeks before decanting and freezing.
One radicle was transferred into 500 μL Cetyltrimethyl ammonium bromide (CTAB) extraction buffer, to which 168 μL 8 M urea and a spatula tip of polyvinylpyrrolidone (PVPP) were added.
Similar(54)
A study published yesterday in the journal Naturwissenschaften describes the fossil and places it in a new, extinct family of harvestmen, based partially on its unusual penis, which sports a spatula-shaped tip.
In microarray experiments with RNA isolated from shoot tips, we observed high expression levels for homologs of SPATULA, a shoot tip transcription factor that is strongly expressed in shoot tips and young leaf primordia [ 36].
Improvements of Pap smear technique, such as the extended tip spatula or the endocervical brush, have aided general practitioners to collect inner cervix cells (Buntinx and Brouwers, 1996).
However, the reduction in cervical adenocarcinoma incidence was not observed in all regions where extended tip spatula was generally used (Visioli et al, 2004).
A second scrape was then taken using the same spatula and the tip, together with adherent cervical cells, was broken off into a sterile container containing 10 ml of storage buffer (0.9% PBS (pH 7.2) +0.1% SDS).
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