We also generated a nanofiber surface with spatially controlled presentation of immobilized PDGF on an aligned architecture, mimicking native tendon tissue.
The sequence and spatially defined presentation of sugars on various oligomers has been elegantly exploited as a tool to investigate the effect of ligand display in binding to cognate protein receptors, such as lectins.
During the press conference, Gilbert said that he plans for "Kraft" to be the first in a series of spatially complex presentations, with Stockhausen's "Gruppen" and Boulez's "Rituel in memoriam Maderna" following in future seasons.
Here, we demonstrate a versatile approach for spatially-defined presentation of engineered proteins within hydrogels through i) immobilization using bio-orthogonal strain-promoted alkyne-azide click chemistry and ii) dynamic protease-driven protein release using exogenously applied enzyme.
Ultimately, these results suggest that BP-mediated photolithography is a facile method to spatially control the presentation of instructive biological cues to cells within CG scaffolds.
siRNA is a potent biomolecule for regulating the cellular behavior in tissue engineering applications, and spatially patterning its presentation to cells may ultimately facilitate the engineering of complex tissues.
Immobilization of cytokines such as LIF and stem cell factor (SCF) to maleic anhydride copolymer thin-film coatings proposed by Alberti and coworkers [ 8] investigated control over stem cell fate through spatially constrained ligand presentation that provides quantitative control of the signaling ligands at material-cell interfaces.
Previous research has demonstrated that number perception influences the allocation of spatial attention, such that the presentation of a spatially nonpredictive number at fixation results in attention being allocated to the left when the central number is low (e.g., 1), and attention being allocated to the right when the central number is high (e.g., 9).
This pattern matches a retinotopic representation of the response field of VS2/3 measured by axonal voltage recordings during presentation of spatially confined stimuli [ 6].
We investigated whether speeded responses to potential emergency driving situations could be facilitated by the presentation of spatially-predictive (80% valid; Experiment 1) or spatially-nonpredictive (50% valid; Experiment 2) vibrotactile cues.
