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Live imaging reveals that FGF becomes specifically concentrated in microluminal structures that assemble at the centre of these organs and spatially constrain its signalling activity.
When placed in solution, the volume fills with electrolyte via capillary action and serves to spatially constrain O2 produced at the photoanode for accurate detection and quantification at the collector [49].
However, appropriately selective small molecule agents are often unavailable, especially for intracellular targets, and pharmacological effects are difficult to spatially constrain at the cellular level.
A-kinase anchoring proteins (AKAPs) spatially constrain phosphorylation by cAMP-dependent protein kinases (PKA).
Although subsequent electron microscopic studies of polysomes formed in translation lysate have supported mRNA pseudocircularization [ 23, 24], recent analysis using electron tomography of polysomes in intact cells has suggested that densely packed, helical array of ribosomes in a large polysome may spatially constrain the interaction of the termini and inhibit circularization [ 25].
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The proposed method consists of four major steps: (1) data pre-processing, (2) initial allocation through spatially constrained cluster analysis, (3) spatial aggregation and peripheral re-allocation of individuals, and (4) an optional hierarchical or non-hierarchical classification to summarize individual patches in few classes.
First, compared to ordinary clustering methods (e.g., k-means and DBSCAN), spatially constrained clustering methods are less sensitive to spatial distance and are more suitable for spatially clustering applications.
In terms of a spatially constrained algorithm, we could identify clusters of high ratings and further explore spatial patterns of clusters of high ratings.
Schoenfelder, S. et al. Polycomb repressive complex PRC1 spatially constrains the mouse embryonic stem cell genome.
Lavrentovich, MO, Wahl, ME, Nelson, DR, and Murray, AW. (2016) Spatially Constrained Growth Enhances Conversional Meltdown.
Craddock, R. C., James, G. A., Holtzheimer, P. E. 3rd, Hu, X. P. & Mayberg, H. S. A whole brain fMRI atlas generated via spatially constrained spectral clustering.
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