These observations suggest that, for the sparsely sampled organisms we examined in this study, there are very few cases of intron loss/gain in single species lineages suggesting that most intron evolutionary events in tetraspanins coincide with major radiations of animals.
This technique has only been sparsely used to examine skeletal muscle (Zuo et al., 2000, 2004), and hence, an initial objective was to examine the reliability of this approach as a means of monitoring intracellular superoxide activities in muscle fibers.
As T-47D cells readily form colonies when sparsely seeded on plastic, we additionally examined whether expression of CCBE1 in T-47DmEcoR cells had a suppressive effect on the colony-forming ability of cancer cells.
We fit a weighted least-squares factor analysis model using Mplus software (version 5.2, Muthén & Muthén, Los Angeles CA) [ 43] to the indicator sets and examined error messages indicating sparsely populated cells, potentially due to logical dependencies.
The olfactory bulbs exhibited no fibers in any species examined (Fig. 16), but sparsely scattered fibers were present in the olfactory cortex and tubercle in all species.
These methods perform very well for structure-rich indoor and outdoor environments, but to the best of our knowledge, their accuracy in sparsely structured open road scenes is yet to be examined.
Although no Cowdry bodies were seen in any samples, individuals from all species except M. discors, P. subtriangulata and D. anus exhibited some of the other features (albeit sparsely and atypically) and 75 samples out of 885 examined were deemed worthy of further investigation.
