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We note, however, that the sparse label in layer 3 of temporal areas exhibited some periodic fluctuation.
Also, the terminal label in area 24 was not uniform along its rostrocaudal extent; the highest density was located rostral to the genu of the corpus callosum (Fig. 4 d), whereas only sparse label was found caudal to the genu (Fig. 4 g).
Finally, the temporal injections all produced extensive label in layer 2 (for 1.5 2.5 mm), a secondary band in layer 6, and scattered Zn+ neurons in layers 3 and 5. None of these intrinsic connections had any discernible patchiness, except that the sparse label in layer 3 of temporal areas showed some periodic fluctuation.
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On the other side, sparse labeling can bleach quickly.
Guttman et al. set up a semi-synthetic depth map method [3] with a sparse labeling depth estimation method.
These lines should prove particularly useful in the context of sparse labeling experiments.
Sparse labeling also requires targeting of postmitotic cells to avoid generating clonally related clusters of neurons.
Sparse labeling is also seen in the thalamus, hypothalamus, cortex and striatum (Figure 1e-g and Figure S1).
Positive areas (arrows) were most numerous in the 24 month-old samples, with only sparse labeling of 6 and 16 month-old samples.
For applications requiring sparse labeling, efficiency can be reduced by decreasing the volume of virus injected, and by directing imaging studies to the periphery of viral transfection.
A major advantage of sparse labeling is that it allows reliable and repeated imaging of dendritic and axonal structures of the same neuron [27].
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