It is most extreme in the southern Terai region, where a 2006 insurrection by ethnic Madhesis has sparked agitations by their neighbours.
Every few years, such shootings spark new agitation against toy weapons.
Sparked initially by agitation against racist hiring practices in Bay Area businesses, it escalated into the Free Speech Movement in response to the university authorities' attempt to crack down on the pamphleteering and recruitment taking place on campus.
Lactobacilli were grown in lactobacilli MRS broth (Difco, Sparks, MD) at 37°C without agitation or anaerobically on MRS-agar plates (BD - GazPak EZ, Sparks, MD).
Her agitation made me agitated.
Each EHEC strain was individually cultured in 10 mL of tryptic soy broth (TSB, Becton-Dickinson, Sparks, MD) at 37°C for 24 h with agitation (150 rpm).
Chopped glands from one animal were then placed in 10 ml digestion mix containing 3 mg/ml of collagenase A (Roche 11088793001, Mannheim, Germany) and 0.67 mg/ml trypsin (Becton Dickinson (BD) 215240, Sparks, MD, USA) at 37°C for 45 minutes with agitation every 15 minutes.
And, most dangerous of all, ethnic violence combining with popular discontent and Islamist agitation in the Ferghana valley linking south Kyrgyzstan, Uzbekistan and Tajikistan, may spark a wider conflagration.
All isolates were grown in Middlebrook 7H9 (BD Diagnostics, Sparks MD) w/OADC (BD Diagnostics), 1% Tween 80 and 10% glycerol under agitation for two weeks before aliquots were stored at −70°C in an equivalent medium containing 10% glycerol.
E. coli and Salmonella strains were grown at 37°C with agitation (100 rpm) in Luria-Bertani (LB) and Tryptic soy (Difco, Becton Dickinson, Sparks, MD), either broth or agar, respectively.
A solo of scrabbling, breathless agitation conveys Victor's terrified excitement, and Macfarlane creates a moment of total theatre in the clamorously sparking "galvanising machine" and the appearance of the Creature – a bare, forked Gollum, criss-crossed with raw and bloody seams.
