Forty-two yeafterfter Allen made his initial observations, Freeman and Wright [16] reproduced and confirmed in rat and dog that midline incision is beneficial in preventing complete paralysis by preserving the spared cord tissue (unharmed by the primary trauma) and its related motor function.
The individual subvolumes of spared spinal cord tissue were obtained by multiplying the cross-sectional areas of spared tissue by the distance between sections, and the subvolumes were added to obtain the total volume of spared spinal cord tissue.
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The volume of spared spinal cord tissue was analyzed by the method of Cavalieri's principle [43].
Quantification data showed that transplantation of F3.VEGF significantly increased the volume of spared spinal cord tissue (p<0.001 compared to PBS or F3 group) (Fig. 6J).
Since the volume of spared spinal cord tissue was different between the groups, all the comparisons of the cell numbers between different groups were based on the density (i.e. the number of cells per 1 mm3 of spared spinal cord tissue) rather than absolute number.
We have used a luxol fast blue staining to examine spared spinal cord white matter following compression.
In addition, transplanted cells also secrete trophic factors, which may have neuroprotective effects and/or promote plasticity in the spared spinal cord.
This cotransplantation strategy rescued a significantly greater percentage of spared spinal cord tissue from contused lesion, and successfully enhanced remyelination after injury.
The size of the SCI was estimated by measuring the area of spared spinal cord tissue at the centre of the lesion (Fig. 7a).
No significant difference was detected between groups 6 and 7 (average spared spinal cord tissue in group 6: 0.88 ± 0.05 mm, in group 7: 0.91 ± 0.06 mm, p = 0.755; percentage compared to intact spinal cord in group 6: 59.52 %, in group 7: 61.16 %).
