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Individual life spans were calculated using the frequency of bees alive at each temporal observation.
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Patent life span was calculated using the announcement date of related patents.
The weight function for each point in the span is calculated using Eq. (5) (Cleveland and Devlin 1988): w_{i} = left( {1 - left| {frac{{x - x_{i} }}{d(x)}} right|^{3} } right)^{3} (5 where x is the observed value we intend to be smoothed, x i is the nearest neighbor of x which is defined within the span, and d(x) is the maximum distance between x and x i.
P-values for replicative life span analysis were calculated using a 2-tailed Wilcoxon Rank-Sum test.
Molar masses spanning elution peaks were calculated using ASTRA v6.0.0.108 (Wyatt).
Genetic distance between SNPs spanning intergenic CSSs and flanking genes were calculated using the recombination rate information and was used for investigating the associations between CSSs and their flanking genes.
Reproductive life span was divided into quartiles based on its distribution in the subcohort, and HRs were calculated using the highest quartile as a reference.
All percentages were calculated using USD.
Gene correlations were calculated using Pearson correlation.
Scores were calculated using equal weights.
Rates were calculated using yearly census data.
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