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The Supercharger network actually spans down both coasts from Seattle to San Diego and Connecticut to the tip of Florida.
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Then beads were spanned down, re-suspended with loading buffer and protein complex was analyzed by western blot.
The sample was span down in an Eppendorf centrifuge at 13 krpm for 10 min to minimize the chance of introducing small magnetic micropaticles, which were not completely removed by the magnet and still were present in the solution.
Protein A/G beads were span down and washed twice with ice cold PBS supplemented with 0.5 mM vanadate.
Cells were spanned down and pellets resuspended gently in 450 μl of sterile ddH2O, 50 μl of 3.5 N NaOH and incubated for 5 min at room temperature.
Villi were then briefly span down, the supernatant removed and RNA extracted from the samples using the RNeasy Plus Mini Kit (QIAGEN, Valencia, CA).
After 6 days, pre-cultured algae were span down by centrifuging at 1,000 g for 3 min and washed once in the targeted culture medium by successive resuspension and centrifugation to eliminate pre-culture medium.
For this purpose, algae were span down by centrifuging at 1,000 g for 3 min, half of the culture medium was removed and algal pellets were resuspended in the remaining volume.
In March of that year floods over-topped it by approximately 10 feet and five of the bridge's seven spans went down, girders were broken, piers sheared through, and some swept into the river.
The lead changed hands six times over a three-minute span down the stretch, with Durant personally matching every Knicks point.
This implies that time-management and multitasking skills are essential--something which suits me and my goldfish-like attention span down to the ground.
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CEO of Professional Science Editing for Scientists @ prosciediting.com