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Work on the project began when co-first authors Ruixuan Gao and Shoh Asano of the Boyden lab decided to image complete neural circuits spanning millimeters or more using expansion microscopy.
Work on the project began when co-first authors Ruixuan Gao and Shoh Asano of the Boyden lab at MIT endeavored to image complete neural circuits spanning millimeters or more using expansion microscopy.
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Neuronal processes in the cerebral cortex can span millimeters to centimeters while in the retina the two synaptic layers span only 60μm, and most lateral processes span only several hundred μm.
Lamellar and fibrous peritectic cooperative growth of the primary α- and peritectic β-phases has been observed on length spanning several millimeters.
That the activity of several discrete regions of visual cortex spanning several millimeters might be correlated is implied by the statistical structure of the visual world, regardless of intracortical connectivity.
The teams tested the ExLLSM technique not only on the entire fruit fly brain, but also on a sliver of mouse brain spanning the millimeter-thick cortex, with similar results.
However, errors only exist in a time interval of tens of milliseconds (several times of particle relaxation time) and a spatial span of several millimeters (several times of particle diameter).
These approaches can characterise structures spanning nanometer to millimeter length scales to define the nanostructure of individual collagen VI microfibrils and the micro-structural organisation of these fibrils within tissues to help in the future design of better mimetics for tissue engineering.
These structures have cross-sectional dimensions that can be tuned from 5 to 500 nm, with lengths spanning hundreds of nanometers to millimeters.
Hybrid models, which use cellular automata, agent-based or multi-cell techniques to represent individual cells and continuum PDEs to represent diffusion of molecules, can capture more detail than continuum models spanning scales from microns to millimeters (for comprehensive reviews of mathematical models of tumor-growth and angiogenesis see [23] [27] and references therein).
Due to the complex structures of living systems, with size scales spanning from the micron to millimeter range, the use of microtechnology to recreate in vivo-like architecture has exciting potential applications.
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