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Then beads were spanned down, re-suspended with loading buffer and protein complex was analyzed by western blot.
Cells were spanned down and pellets resuspended gently in 450 μl of sterile ddH2O, 50 μl of 3.5 N NaOH and incubated for 5 min at room temperature.
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The Supercharger network actually spans down both coasts from Seattle to San Diego and Connecticut to the tip of Florida.
Villi were then briefly span down, the supernatant removed and RNA extracted from the samples using the RNeasy Plus Mini Kit (QIAGEN, Valencia, CA).
Daniel Lickess, 29, had been "showing off" in the Subaru Impreza when he lost control and it span down the road in Brighouse, West Yorkshire, in June.
The sample was span down in an Eppendorf centrifuge at 13 krpm for 10 min to minimize the chance of introducing small magnetic micropaticles, which were not completely removed by the magnet and still were present in the solution.
Protein A/G beads were span down and washed twice with ice cold PBS supplemented with 0.5 mM vanadate.
After 6 days, pre-cultured algae were span down by centrifuging at 1,000 g for 3 min and washed once in the targeted culture medium by successive resuspension and centrifugation to eliminate pre-culture medium.
For this purpose, algae were span down by centrifuging at 1,000 g for 3 min, half of the culture medium was removed and algal pellets were resuspended in the remaining volume.
Her connections spanned Buckingham Palace, Downing Street, Hollywood and the Kennedys.
Once the leader of a drug empire that spanned five cities up and down the East Coast, Brian was gunned down in Baltimore in 1995.
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