Diagrams and pictures employ two dimensions, and models employ three; but writing is partially relieved of memory-span restrictions by the permanence of visual marks.
Oligonucleotides harboring the mutant sequence(s) were used in amplification reactions with either upstream or downstream oligonucleotides spanning unique restriction sites (Table SI).
Only 9% of the contigs spanned the restriction fragments.
Such di-tags are assumed to comprise a contig spanning several restriction cut sites, and so are discarded.
It would also extend the life span of import restrictions to ten years from five and grant the President the power to impose emergency restrictions on the importation of cultural artifacts from any country in the world, like Afghanistan, not just Unesco convention parties.
If the fishing restrictions span these patches of habitat, that could limit the "spillover" into the waters still plied by fishing boats.
Mutation in Sir2, a gene necessary for asymmetric segregation in yeast [8], resulted in a longer chronological life span under caloric restriction [18].
An increase in autophagy controlled by mTOR extends life span via dietary restriction and insulin signaling.
Polymerase chain reaction (PCR) primers were designed to span a DraI restriction fragment length polymorphic site in the gene.
The quintessential microbial aging model, S. cerevisiae, is a commonly used organism for life span and calorie restriction studies for a multitude of different reasons.
Overexpression of Sir2 genetically, or increase in Sir2 activity mediated by the drug resveratrol, increases life span without calorie restriction in yeast, nematodes, fruit flies and fish [ 13, 17, 18].
