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Thus, both in terms of UV-insensitivity and in FRET span of the sensor Venus proves a much better FRET acceptor.
Because the initial proposed life span of the sensor was 72 h, those children who had it in place greater than this period form the basis of this analysis.
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Both show a 44% increase to the already robust FRET span of the originally published sensor [1] and in addition are much more refractory to pH changes and UV illumination.
Whereas the cAMP-induced FRET change in CFP-Epac-YFP is already quite robust (CFP/YFP emission ratio changes by ∼30%), the recent introduction of a range of new fluorescent proteins prompted us to further optimize FRET span and other properties of the sensor by systematic variation of the donor- and acceptor fluorescent proteins.
The most promising pairs were subsequently used in an attempt to improve the FRET span of the Epac-based cAMP sensor.
Therefore, to clarify these factors, energy efficiency is defined as the number of data packets delivered from the percentage of alive source or intermediate nodes to sink with optimal spanning over the lifetime of the sensor node in the network [43].
We did this to ensure that each study participant had time to calibrate and adjust to the device, to minimize the number of sensor failures in the data more likely to occur toward the end of the sensor life span, and to obtain a comparable number of glucose readings for each period of RT-CGM use for each participant.
It results in redundancy of sensed data and excessive forwarding of packets in network which ultimately results in unnecessary energy expenditure reducing the life span of sensor nodes.
As outlined before, spectral overlap of CFP and YFP complicates quantitative FRET measurements and it diminishes the FRET span of ratiometric sensors.
It builds a tree that spans all the sensor nodes.
(i) : the father of the sensor in the final spanning tree.
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