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Instead it prolonged the life span of cells.
Cells have a limited life span to begin with, and thus they are programmed to die some time after differentiation (the life span of cells varies according to type; some white blood cells, for instance, live for hours, whereas certain neurons live for decades).
Moreover, depletion of p300 increases the proliferative capacity of HFKs, extends the life span of cells and allows differentiated HFKs to re-enter the cell cycle.
Mutations in critical residues of the chromodomain (F11A and W32A, Figure S2) or deletion of the Pc-box (ΔPc) inhibited the ability of CBX7 to extend the life span of cells [20].
10.7554/eLife.01883.010 Figure 8. Yos9 deletion shortens the life span of cells.
The ectopic expression of hTERT has been reported to extend the life span of cells [ 28].
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In cases where granule cells could be observed forming a bridge across a region devoid of pia (fused molecular layers), we observed TH-labeled axons that appeared to span this bridge of cells– 7(c)).
In cases where granule cells could be observed forming a bridge between folia VIII and IX, we observed 5HTT-labeled axons that appeared to span this bridge of cells (data not shown).
These facts suggest that a stochastic process is involved in establishing the finite life-span of cells in culture, but this process is not related to telomere erosion as a function of the number of cell divisions.
Transfection with LT extended the life span of OSE cells, but, these LT-transfected OSE cells (OSE+LT) underwent senescence in 9±2 passages.
A more recent study used conditional gene targeting to ablate B lymphopoiesis in the BM in order to determine the life span of B cells in the absence of B cell influx from the BM.
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