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Conclusion Placing the spacer immediately upstream from the cannula was the most efficient configuration for pMDI salbutamol administration in an HFT circuit, actuation being synchronize with inspiration.
The putative origin in the Stigeoclonium cpDNA was located in the trnS gga)- rrs spacer, that is, in the spacer immediately adjacent to that carrying the putative origin in the Schizomeris cpDNA.
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The presence of structured spacers immediately suggest that dimers may fold into stable hairpins.
The proximal 3 kb spacer regions immediately upstream from the translation start site that harbor promoters and the majority of known transcription factor sites in humans were analyzed separately.
Recent work by Yang et al. (2008) combined a furin cleavage site and a V5 oligopep-tide spacer sequence immediately upstream of 2A [ 54].
Consistent with the WebLogo analysis of the consensus sequence and analyses by an EMSA and ITC, mismatches at the edges of the motifs had a weaker impact than those in the middle of the motif on the binding affinity, and a GC-rich sequence in the spacer or immediately following the second motif significantly reduced the binding affinity.
When the spacer was placed immediately upstream from the cannula, the masses collected were 11.8, 10.7 and 6.2%% of the nominal dose for HFT flow rates of 30, 45 and 60 L/min, respectively, with the quiet breathing setting.
The spacer and the sequence immediately following the second motif are AT-rich.
The short (3 or 4 nucleotides) proto-spacer adjacent motifs (PAMs) located immediately downstream of the proto-spacer appear to determine the selection of the protospacer followed by integration into a pre-existing CRISPR array [ 11, 12].
After 5 s of growth interruption, the GaAs spacer was grown at 540 °C immediately.
It has been reported that in γ-retroviral constructs, expression of multiple genes linked with F2A peptides was facilitated by a spacer sequence (-SGSG- or -GSG-) immediately upstream of F2A22 [ 12, 34], while Yang and colleagues [ 24] tested both these spacers plus the V5 tag which acted, in this context, as a spacer.
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