Sentence examples for spacer guide from inspiring English sources

Spacer (guide) sequences for SpCas9 and St1Cas9 have been described previously59,60 and are provided in Supplementary Table 3. Untagged AcrIIA proteins were expressed transiently from a modified pVAX1 vector (Thermo Fisher Scientific) containing a beta-globin intron.

The finite element (FE) model of spacer grids, guide thimbles with dash-pots & flow holes, in addition to spot welds between spacer grids and guide thimbles, has been developed using Shell 181 element.

The crRNA consists of a conserved repeat sequence and a variable spacer sequence (guide) complementary to the target gene sequence (Brouns et al. 2008; Hale et al. 2009).

And present spacers can guide the acquisition of new spacers from the same DNA molecule [ 45].

When multiple probe arrays are utilized, a mechanical guide (spacer) is employed to maintain proper spacing and alignment.

Here we show that engineering a hairpin secondary structure onto the spacer region of single guide RNAs (hp-sgRNAs) can increase specificity by several orders of magnitude when combined with various CRISPR effectors.

Occasionally, the regions were found in the spacer region between the guide and C or C′ box (e.g., snR47 and snR60).

The enzyme is active in a wide variety of organisms and is also quite specific, cutting only protospacers that are nearly identical to the spacer sequence of the guide RNA (Hsu et al., 2013; Mali et al., 2013a; Pattanayak et al., 2013).

However, the sequences of most of these Cas proteins are so divergent between the two species (e.g. 28% identity for Cas3) that it is noteworthy that the G. sulfurreducens system seemingly required no context other than its cognate repeat to recognize spacer #1 as a guide and hisS of P. carbinolicus as a target.

The programmable pre-crRNA, which contains nuclease guide sequences (spacers) interspaced by identical direct repeats, is processed to mature crRNA in combination with tracrRNA.

Optimal design of deflector for spacer grid of the TVS-KVADRAT fuel assembly in the standard cell in the area of guiding channels was identified.