Values represent the sum of MS2-aligning spacers across replicates, n = 64 technical replicates from n = 2 biological samples, representing 22 million spacers.
Repeats were removed to determine the homology of spacers across strains, and the CRISPR spacer array was manually aligned to optimize the homology of spacers across Salmonella strains.
Further analyses of 32 gadiform species, representing 8 families and 28 genera, revealed the evolutionary preserved presence of T-P spacers across all taxa.
The horizontal black bars are mean genome-aligning spacer count across all transcripts ± s.e.m.m
When the original hybridization blots were checked, an irregular signal distribution for spacer 15 across all samples was noted.
For each set, we computed the summed fraction of randomly chosen spacer groups across different subjects and nucleic acid types, and from those computed an empirical null distribution of statistics.
Furthermore, by comparing the CRISPR spacer sequences across body sites and among individuals, they were able to demonstrate that, while resampling of the same site from the same individual recovered many of the same spacers, different individuals had almost no spacers in common.
The propensity of hypervariable CRISPR loci for HGT [ 75] is also illustrated within L. casei by the co-occurrence of various IS elements for both types of loci, the sharing of contiguous spacer sets across strains that belong to different phylogenetic clusters, and the skewed GC content of cas genes 50-633% for Ldbu1 cas genes versus 46.5% genome-wide content).
a, Nucleotide probabilities determined using plasmid-aligning spacers merged across n = 14 independent biological samples, prepared as for Fig. 2f.
Correspondence analysis of the 265 peaks in the automated ribosomal intergenic spacer analysis profile across the 188 samples revealed that the first 2 components contributed 7.1 and 3.8% to the total variation in the profile.
