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In vivo [3H]-nicotinate uptake by the retina was 5.4-fold greater than that of [14C]-sucrose, a BRB impermeable vascular space marker.
Spreading of [C]inulin, an extracellular space marker, was unaffected by activation, suggesting that glutamine and lactate may have diffused, in part, through intracellular routes.
Uptake studies can be performed with two radiotracers: a test molecule and a reference molecule which serves as an extracellular space marker.
Under the conditions used, about 95% of the intermembrane space marker adenylate kinase was lost (data not shown), implying efficient mitoplasting, whereas recovery of the matrix marker citrate synthase was close to 100%, showing that mitoplasts were not disrupted.
We did not observed a change in vascular permeability in nicotine pre-exposed sham animals compared to saline infused sham group as measured with vascular space marker [C]-sucrose in the perfusion medium.
The latter is usually radiolabelled mannitol or sucrose or a larger extracellular space marker like inulin, the choice depending on the size and physical properties of the test molecule.
[H]cortisol (3.6 n m) or [H]corticosterone (3.8 n m), along with [C]sucrose (vascular space marker, 0.5 1.0 n m), was then administered by a slow-drive syringe pump (model 22; Harvard Apparatus, Kent, UK) into the artificial plasma.
This can be done by using equally spaced markers to perform a global linkages search of the whole genome.
The LD panel with markers selected on the basis of their effects always performs better than the LD panel with evenly spaced markers.
The trade-off between the LD panels with evenly spaced markers and selected markers must be considered, which depends on the number of target traits in a breeding program and the genetic architecture of these traits.
