In contrast, when fru+ neurons are silenced, deposition of successfully fertilized eggs is still observed [ 15], suggesting that different subsets of the dsx+/ fru+/ ppk+ SP-responsive sensory circuit may direct distinct postmating behavioral responses.
Recently, a SP-responsive G protein-coupled receptor for SP-mediated postmating responses has been identified [ 13].
A SP-responsive G protein-coupled receptor for SP-mediated postmating responses has been identified; females lacking this sex peptide receptor (SPR) remain receptive, exhibiting virgin-like behaviors, postmating [ 51].
Expression of a membrane-bound form of SP (mSP) in the CNS has been shown to be sufficient to induce postmating responses in virgin females and has been used to identify SP-responsive cells and tissues [ 15, 18].
These results suggest that SP-responsive dsx neurons may be cholinergic.
We uncovered shared circuitry between dsx and a subset of the previously described SP-responsive fru +/ ppk +-expressing neurons in the reproductive system.
We demonstrated that two dsx clusters, composed of three bilateral neurons of the uterus, comprise a more defined component of the SP-responsive sensory circuit.
The suppression of postmating responses via disruption of dsx neuronal function [ 4] also suggested an overlap between dsx and the SP-responsive fru +/ ppk +-expressing neurons in the reproductive system.
Our results show that in the female, dsx neurons associated with the internal genitalia not only form a component part of the previously described fru+/ ppk+ network, but in fact define a more minimal SP-responsive neural circuit capable of inducing postmating changes, such as reduced receptivity, increased levels of rejection, and egg deposition.
We found the QL-SP to be a responsive and valid measure in the study despite it being developed originally for the partners (spouses) of MI patients [ 60].
SP programs need to be responsive to local needs.
