Sentence examples for sp buffer from inspiring English sources

After His6-TEV cleavage at 4°C overnight, the sample was diluted in SP buffer A: 30 mM HEPES pH 7.4, 3 mM BME and then loaded onto a HiTrap SP HP 5 ml column (GE healthcare, Piscataway, NJ).

Columns were washed using 5 mL of SP buffer with 40 mM imidazole.

Apohydrogenase was eluted using 5 mL of SP buffer with 250 mM imidazole.

Pooled fractions were dialyzed twice for 3 hr each time against SP buffer with 10% vol·vol−1 sucrose.

50 mM sodium phosphate buffer, pH 7.4 with 100 mM NaCl (SP buffer) was used for Ni+2-affinity chromatography.

Cell suspensions were incubated at 23°C for 30 min and then diluted with 5× SP buffer (10 mM imidazole final concentration).

Two populations of His6 ComFB could be separated by a linear gradient of SP-elution buffer (SP-binding buffer with 1 M NaCl).

A five-CV linear gradient up to 35% SP-elution buffer separated impurities from a first population of ComFB, which began eluting at 35% of elution buffer.

The pH was then lowered from pH 8.0 to 6.0 by drop-wise addition of the protein solution into SP-binding buffer [50 mM BisTris (pH 6.0), 50 mM NaCl, 2 mM BME, 5% glycerol], with a final dilution factor of 1 5.

The eluate was diluted in buffer SP-A (20 mM HEPES-KOH [pH 6.5], 10% glycerol, and 0.02% CHAPS) and concentrated repeatedly to reduce the salt before loading to SP-Sepharose.

After the salt was reduced by repeated dilution in buffer SP-A, the sample was fractionated on 2 ml ceramic hydroxyapatite (CHT) column (Bio-Rad Laboratories InCA, Hercules, CA) with a linear gradient of KH2PO4 K2HPO4 0 3000 mM) in buffer CHT-A (5 mM KH2PO4 K2HPO4, pH 7.0, 150 mM NaCl).