To acquire cell free supernatant extract, a loop full culture of Streptomyces sp. GUT 21 was inoculated in soybean meal medium for production of secondary metabolites [35].
The lipid emulsions are based upon soybean oil, medium-chain triglyceride (MCT) oils,olive oil, fish oil, and a mixture of these oils (Table 1).
After a 24-h period for radioactive decay, two 100-μL aliquots were taken from each sample and mixed with soybean casein digest medium and fluid thioglycollate medium, respectively, and incubated for 14 days at 37 °C.
The concentration of barley flour, corn flour and soybean flour in medium were significant factors.
(Abdel-Mawgoud et al. 2009) reported the production of a rhamnolipid by Pseudomonas aeruginosa isolate Bs20 on soybean oil amended medium.
Particularly, Bacillus subtilis produces a large amount of iturins, cyclic lipopeptide antibiotics, in soybean curd residue medium (Ohno et al. 1995, 1996).
The condensed soy protein medium (g/L) consisted of starch 35, dextrin 5, soybean meal 30, condensed soy protein 7, ammonium sulfate 3.0, NaCl 2.0, Cand3 7.0, antifoamfoagentent 1.9 mL.
Soybean-casein digest medium was purchased from Becton, Dickinson and Company (Franklin Lakes, NJ, USA).
S. aureus was grown in soybean-casein digest medium under aerobic conditions at 37 °C until late log phase.
