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Because of the limitations of "conventional" markers, there has been a search for additional sources of specificity so as to expand the target pool of cancer-associated molecules.
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Interestingly, the AT-tracts in PRRII are separated by less that 10 bp which suggests that the observed negative supercoiled DNA structure is the source of specificity for HMGA1 positioning and high affinity binding.
Position 4a is a well-documented source of specificity for Mcl-1 binding.
Given that the overall fold of all lipoyl domains is identical, the source of specificity of the kinases for the lipoyl domains is not obvious.
But as the mitotic rate of white cells is often higher than that of tumors, the efficiency of chemotherapy usually relies on a second source of specificity.
Reviewer's comment to the authors' response: BRENDA is not the only source of specificity data and I did not intend to imply that it was.
Importantly, the source of specificity of interactions between cohesin/CTCF sites is still unresolved, as it may be either driven indirectly by various regulated processes, such as transcription and replication, or regulated directly via additional uncharacterized mechanisms.
Although the underlying reason for this source of specificity is not yet understood, it is clear that the two aa-tRNAs tested interact with EF-Tu in a thermodynamically distinct manner using contacts throughout the interface and are not limited to the regions where the two tRNA sequences differ.
Site-directed mutagenesis elucidated the sources of enzymatic specificity.
Nevertheless, the associations between specific tumour types, genes, pathways and processes are a very interesting source of information in the search for biological sources of tumour specificities.
B cells expressing IgE contribute to immunity against parasites and venoms and are the source of antigen specificity in allergic patients, yet the developmental pathways producing these B cells in human subjects remain a subject of debate.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com