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Interestingly, the AT-tracts in PRRII are separated by less that 10 bp which suggests that the observed negative supercoiled DNA structure is the source of specificity for HMGA1 positioning and high affinity binding.
Position 4a is a well-documented source of specificity for Mcl-1 binding.
Reviewer's comment to the authors' response: BRENDA is not the only source of specificity data and I did not intend to imply that it was.
Given that the overall fold of all lipoyl domains is identical, the source of specificity of the kinases for the lipoyl domains is not obvious.
But as the mitotic rate of white cells is often higher than that of tumors, the efficiency of chemotherapy usually relies on a second source of specificity.
Importantly, the source of specificity of interactions between cohesin/CTCF sites is still unresolved, as it may be either driven indirectly by various regulated processes, such as transcription and replication, or regulated directly via additional uncharacterized mechanisms.
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Because of the limitations of "conventional" markers, there has been a search for additional sources of specificity so as to expand the target pool of cancer-associated molecules.
We have recently shown that diverse N-substituted residues (peptoids) can replace the prolines in the PxxP motif, yielding a new source of ligand specificity.
Library WySH2A was constructed to examine the potential for synthetic complementarity-determining region (CDR -H3 diversity to aCDR -H3he lone source of bindiversityificito.
While the LSPs that distinguish cattle and sheep MAP isolates represent a potential source of species specificity, the lack of LSPs amongst the cattle genomotype isolates suggests that other mechanisms may also have an influence.
The single-pot antibody library PHILOtop may thus represent a useful source of binding specificities, facilitating preclinical studies in immunocompetent syngeneic mouse models of pathology.
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