Sentence examples for source of polymerase from inspiring English sources

This study raises a number of questions: 1) The source of polymerase the authors used is micrococcal nuclease treated vRNPs purified from virions.

This result is represented as Figure 4 figure supplement 2, and explained in the revised manuscript (paragraph two, "Interaction between IREF-2 and viral RNA polymerases").Reviewer #3: 1) The source of polymerase the authors used is micrococcal nuclease treated vRNPs purified from virions.

In situ analysis of such cases, however, may reveal occasional EBV-positive lymphocytes admixed with the EBV-negative tumour cells as the source of the polymerase chain reaction signal.

For all PCRs, Phusion Flash PCR master mix (Thermo Scientific) was used as a source of proofreading DNA polymerase.

The following two bacterial strains harboring λDE3 lysogen (a source of T7 RNA polymerase) were used for generating dsRNA from the recombinant plasmids: BL21 DE3): ompT hsdSB (rB-mB) gal dcm (Novagen) - a strain deficient in lon and ompT proteases and used for efficient production of recombinant proteins.

Mycobacterium tuberculosis H37Rv (M. tuberculosis) strain was used as a source of genomic DNA for polymerase chain reaction (PCR) to amplify mfd gene.

Thus, transfection of cells with VPg-linked sgRNA alone allows the analysis of virus translation in the absence of replication as there is no source of the NS7 RNA polymerase.

Many scientists are familiar with Thermus aquaticus, a thermophilic microbe that can survive temperatures upwards of 70°C, the source of the first thermostable DNA polymerase, Taq.

To determine where the block in PCR amplification occurred, we performed primer extension assays using two commercial sources of Taq DNA polymerase.

PCR reactions were set up according to the manufacturer's instructions using two commercial sources of Taq DNA polymerase (Roche and Sigma).

Other factors that affected cloning success included the source of amplification primers and the use of polymerases with different fidelities (see Table 1).