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Also congruent with Baums et al. (2006), colonies were larger at the coastal sites that exhibited clonal structure compared with the offshore sites (Table 1); this may provide a source of fragments if exposed to physical disturbances that are not strong enough to completely break up the large colonies.
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The one-factorial analyses test for source effects of fragments originating from the ' Back Reef' (B), ' Upper Slope U(U) and ' Deep Slope' (D) habitats transplanted to the ' Deep Slope' habitat.
As reported earlier, 2-methylpentane was the main source
Key choices need to be made on both the selection criteria to be applied and the source of the fragments in the library.
This is due to the oxidation of the source of Zn fragments which is a well-known drawback in the synthesis of MO NWs by the reactive vapour transport method where the metal constituent is included in situ.
We used pUC19 DNA digested with different restriction enzymes as a source of DNA fragments for religation and amplification.
To test its viability as a source of antibody fragments, the entire library was subjected to four rounds of selection using several model haptens, proteins and viruses.
However, it may in addition be a functionally important molecule in its full-length configuration, as well as be the source of numerous fragments with varying effects on neural function [ 45].
A better approach is the one implemented in ClonalOrigin (Didelot et al. 2010), where the source of recombined fragments is inferred jointly with the recombination events rather than relying on a postprocessing step.
Measuring only the ARGS neoepitope, we are, however, unable to ascertain the source of aggrecan fragments being degraded, which could span everything from newly synthesized aggrecan not incorporated into a functional matrix to C-terminally truncated aggrecan resident in the cartilage for a long period [ 39].
Three to eleven recombinant plasmids per PCR fragment were subjected to bi-directional sequencing allowing for repeated isolation of identical clones from different sources of PCR fragments corresponding to about 660 sequencing reactions (Table 1).
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