Exact(8)
(B ) Sequence and secondary structure of a proof-of-concept RNA with one radical source attachment site (U13, in green).
Conceivably, by placing the receptor closer to an NO source, attachment of the α1β1 isoform to the outer cell membrane could perform a similar function.
We have edited the text regarding the reverse transcription-terminating modifications to indicate this (Results section): "However, we were surprised to find additional stop sites beyond these source attachment positions a model hairpin RNA with a single radical source attachment position".
As described in the main text, unsatisfied constraints could arise from structural perturbations due to radical source attachment, or conformational flexibility of the RNA, or the benzyl-thiourea linker tethering the radical source to the RNA backbone.
This expression simplifies in the limit of no background processes and low-oxidative damage rates (including radical-induced strand scission and nucleobase oxidation) (p i s, q j s ≪ 1, except at p i s = i = 1, corresponding to a reverse transcriptase stop at the site of radical source attachment).
This high rate of stops would have been difficult to quantify in prior gel and capillary electrophoresis (CE) measurements (Mitra et al., 2008; Kladwang et al., 2014); the rate and proximity of these stops to the radical source were confirmed through both CE and next-generation sequencing tests on a model hairpin RNA with a single radical source attachment position.
Similar(51)
Computers are usually affected through rogue emails (from friends or from banks, government or official source) containing attachments.
The wall has been a site for Jewish prayer and pilgrimage for centuries; the earliest source mentioning Jewish attachment to the site dates back to the 4th century.
We also showed E. anophelis NUHP1 and E. meningoseptica ATCC13253 have distinct response toward iron sources during cell attachment and biofilm formation.
Plants, in contrast to animals (e.g., geckos) are only a limited source of inspirations for attachment mechanisms.
Within all combinations of strain, egg source, and temperature, the attachment levels after 1 min were lower than after 20 min.
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