It is really fast and offers some nice ways of sorting with tags.
Cell apoptosis was mainly determined by fluorescence-activated cell sorting with Annexin-V and 7-aminoactinomycin D staining.
Cluster analysis was performed with the TIGR MultiExperiment Viewer (MeV) software (J. Craig Venter Institute; Rockville, MD) using a k-means sorting with the default parameters.
Sorting with the fundamentals-oriented ranking system not only makes the list size more manageable but also improves performance, turning $1,000 into $3,249.
Hence while sorting with the keyword proximity score, image descriptions with equal number of keywords are sorted together and then displayed in descending order of their keyword proximity scores, while maintaining the previous structure of decreasing number of keyword occurrences.
Sperm membranes responded to sorting with an increase in the percentage of YoPro-1 positive cells, suggesting the sorted spermatozoa had a reduced energy status that was confirmed by measuring intracellular ATP content.
While this method is far less efficient than sorting with soluble envelope proteins, it isolated broadly neutralizing anti-HIV-1 antibodies that bind cell surface-expressed gp160ΔcBaL but not soluble envelope proteins.
In the case of sorting with the MoFlo cell sorter, prior to cell sorting and in keeping with the biosafety guidelines, CD4+ T cells were fixed with 1% PFA (experiments shown in Fig. 2A).
When hCB CD34+ cells were transduced with MIN-R1 and MIN-210 and purified by immunomagnetic cell sorting with anti-NGFR beads (Miltenyi Biotech) 2 days after transduction following manufacturer's instructions.
CD8 T cell lines were generated after sorting with immunomagnetic beads coated with either pp65495 503/A*0201, BMLF1259 267/A*0201, or BZLF154 64/B*3501 multimeric complexes.
Embryos were collected from established lines and YFP expression confirmed by sorting with the COPAS Select.
