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For all cell types examined, a 2- to 3-fold decrease in size overlap was found when sorting was based on SSC-A rather than FSC-A.
Sorting was based on forward scatter and side scatter properties (FSC/SSC) and surface marker-positive fluorescent gates; CD3 for T lymphocytes; CD19 for B lymphocytes; CD14 in combination with CD16b to distinguish monocytes and neutrophils.
Here, sorting was based on either the p-values of a statistical test in a pair-wise comparison (supervised method) or the contribution to a selected SVD component (unsupervised method).
When sorting was based on the area of the signal (450/50-A) the quality of the size separation (estimated by the Δ median and the percent overlap between the two size distributions) was between 1.7-fold and 8-fold better than the separation achieved by the signal height (450/50-H) or the signal width (450/50-W) for all three cell types (Table 1).
Spike sorting was based on waveform shape differences, peak-to-peak spike amplitudes plotted in principal component space, characteristic inter-spike-interval distributions, and a maximum 1% of spike collisions assuming a refractory period of 1 ms. Candidate spikes with signal-to-noise ratio lower than 2.5 were discarded.
The thematic sorting was based on a content analysis of an initial collection of 300 articles.
Similar(51)
Immigrants' sorting is based on both observable and unobservable factors, which makes it generally difficult to study the effect of negative attitudes on migration decisions and labour market outcomes.
Breathiness is generally treated as a continuum that is difficult to separate into "breathy" and "modal" whether sorting is based on perceived quality, acoustics, or the underlying glottal configuration [10].
A number of techniques for cell sorting are based on electric interaction.
At present, the most commonly used method for human beta and non-beta cell sorting is based on beta cell labelling with Newport green [ 21].
With the rise of flow cytometry and flow cytometric cell sorting, early attempts for flow cytometric cell sorting were based on the strong sideward scattering of HSC due to the specific intracellular (retinol) droplets [ 10].
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