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We believe that the working principle of the current device is applicable to smaller devices; therefore, it may lead to a sophisticated technique of "electrostatic nano-tweezers" for sorting, separation, trapping, and manipulation of individual charged nanoparticles, such as proteins and DNA, which are often naturally charged.
After migration, epithelial cells were purified from PMN by magnetic-activated cell sorting separation using anti epithelial cell adhesion molecule antibodies.
CD14+ cells were purified from the resulting PBMCs using micro beads and a magnetic cell sorting separation unit (Miltenyi Biotec, Bergisch Gladbach, Germany).
MSC can be directly isolated from bone marrow aspirates based on their ability to adhere to plastic when plated in monolayer culture, by flow cytometry and sorting separation or with micromagnetic beads by using specific antibodies for stem cells [ 4].
Flow cytometry allows the analytical, and physical (by fluorescently activated cell sorting), separation of infected macrophages and therefore this population can be separately assessed for immune regulatory changes.
Dielectrophoretic techniques allow for sorting, separation, and isolation of cells from different organisms and tissues.
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Effective design of machines for particle separation, sorting, cleaning and grading also requires a good knowledge of particle dynamics.
The interactions between particles and the analysis of the inter-playing forces in a moving air are essential in the separation, sorting, cleaning and grading processes.
Separation, sorting, cleaning and grading processes mostly exploit the differences in the physical and aerodynamic properties of particles, especially size, shape, density and terminal velocity.
This phenomenon has been widely used for concentration, manipulation, separation, sorting, and transport of particles such as beads, bacteria, and cells [3 5, 9 12].
A magnetic-activated cell sorting (MACS) separation column system (Miltenyi Biotec, Bergisch Gladbach, Germany) was assembled by placing a MiniMacs separation column into the magnetic component and was washed with 500 μl of depletion buffer.
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