Using monoclonal antibodies specific for the TCRBV families expressed by the 50 most frequent TCCs in L-III we sorted previously PHA-expanded CSF-infiltrating T cells from the same patient, cloned them by limiting dilution and sequenced the CDR3 region (Fig. S3).
Cell cycle profile was assessed by fluorescence-activated cell sorting as previously described [46].
These modified forms of Robo were tested for their sensitivity to Comm in the sorting assay previously described (Figure 1B).
Cells were kept on ice in 0.5 μM EDTA and 0.05% BSA in PBS for sorting as previously described (Rock et al., 2007) and sorted on a FACSAria II (BD Biosciences).
To this end, we used the blast culture system to produce pure populations of differentiating cells by cell sorting as previously described (Lancrin et al., 2009; Lichtinger et al., 2012).
He remembered seeing chain-link "cages", of the sort previously described to the Guardian by attorneys and former police superintendent Richard Brzeczek.
Moreover, we show that relationships of the sort previously suggested to occur only among pair bonds outside of the primates (Dunbar & Schultz 2007) may also occur among unpaired ravens and provide additional support for convergent cognitive evolution in birds and mammals (Emery & Clayton 2004; Emery 2006).
All samples were flow cell sorted as previously described and analyzed with a custom SNP array comprising several chromosomal regions previously reported to be implicated in colorectal cancer progression [ 28].
Regions sorted into previously defined communities, such as the fronto-parietal and cingulo-opercular control networks, and the default mode network.
CD45.2+Lin-cKit+ScaI−MPs were sorted at previously described and infected with concentrated virus in 8 μg/ml polybrene by spin infection at 1800 rpms for 45 min.
