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Sorting on a Massively Parallel System Using a Library of Basic Primitives: Modeling and Experimental Results.
After washing, these cells were filtered through a 40 μm cell strainer (JET Biofil, China) to obtain single cell suspension for sorting on a BD FACSARIA III (BD Bioscience, NJ, USA).
We combine color-coding and sorting on a given attribute to reinforce the perception of grouped leaves of similar values.
The dissociated hESCs (adjusted to 106 cells/ml) were stained with SytoxGreen (Invitrogen) or 7-AAD (7-amino-actinomycin D, BD Bioscience) prior to sorting on a FACSVantage™.
On specified days of co-culture, cells were recovered from the culture dishes for analysis and sorting on a flow cytometer.
Cells were filtered through a CellTrics 50 µm filter (Partec) before sorting on a MoFlo high-speed cell sorter (Beckman Coulter).
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At Memphis airport, the parcels were sorted on a table.
Samples were acquired on a Fortessa (BD-Bioscience) or sorted on a MoFlo ASTRIOS (Beckman-Coulter).
Cells were analyzed and sorted on a FACS ARIA II (BD Biosciences).
Lastly, the ZsGreen1-positive cells were sorted on a FACSAria II flow cytometer (BD Biosciences).
Cells were then sorted on a BD FACS ARIA IIIu system using CD14 positive selection.
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