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But he noted that it did not prohibit artificial insemination -- merely the sorting of sperm.
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I sort of agree.
The hybridization method will be useful for evaluating the ratio of X- and Y- bearing spermatozoa in a sperm sample and consequently can be used to evaluate the efficiency of sperm sorting by different techniques such as flow cytometry.
In this study, we tested different protocols for the handling of Iberian red deer spermatozoa before sorting by using different concentrations of sperm (400 or 800 × 106) and adding or not Hoechst 33342 before the transport of samples to the sorting facilities.
The method is based on the separation of sperm using flow cytometric sorting to sort fluorescently (Hoechst 33342) labeled sperm based on their relative content of DNA within each population of X- and Y-spermatozoa.
Instead, using a high-technology method to sort the sperm of dairy bulls, they could produce mostly female calves to be raised into profitable milk producers.
But imagine the sort of impact Ruthless Sperm would have on your co-worker's four-year-old if you forced it on him after his mommy-mandated dance lesson?
Although sperm of all species can be sorted with high purity, achieving pregnancies with the low numbers of sperm needed for commercial application remains particularly elusive in swine.
Analogous results obtained comparing subpopulations sorted by Annexin-V MACS were controversial [36], [37], suggesting that fractionation of sperm according to mitochondrial activity leads to more homogeneous subpopulations.
In this context, dry mass-based sorting of X- and Y-chromosome-bearing sperm has already been attempted [19], and the demand for improvements of this technology in animal husbandry may, appropriately, provide cross-fertilization that will benefit cell and systems biologists.
Particularly in the case of sperm, nanomaterial-based approaches have been shown to improve the efficacy of existing techniques such as sperm-mediated gene transfer, loading sperm with exogenous proteins, and tagging sperm for subsequent sex- or function-based sorting.
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