Sentence examples for sorting and functional from inspiring English sources

Unmodified wild-type receptor cDNA was subjected to successive cycles of mutagenesis and fluorescence-activated cell sorting, and functional expression could be increased for all three GPCR targets.

We conclude that two important biological causes of discordance among orthologous genes are incomplete lineage sorting and functional restriction.

In humans, terminal end bud structures are not as prominent and identifying stem cell zones has had to rely on microdissection followed by cell sorting and functional characterisation of putative cells to determine stem cell niches in ducts and lobules.

This illustrates the power of combining index sorting and functional outcome in more variable cell systems and sets the stage for other groups to use the technique in their studies to improve purity and link molecular states with functional outcomes.

Differential cell counts of BALF cells, assessment of T cell responses in MLNs and lung, flow cytometric analysis, sorting and functional analysis of lung CD11c+ DCs were all performed as described in the Supporting Information Materials and methods section.

We demonstrated how VRC01-class antibodies emerged in a Chinese donor by antigen-specific single B cell sorting, structural and functional studies, and longitudinal antibody and virus repertoire analyses.

PredictProtein offers rich information about the local sequence features of a protein, such as the secondary structure, transmembrane helices, protein sorting signals and functional sites.

Due to lower expression of CD44 in luminal cells, compared with basal cells, we used the relative CD44high/CD24low of Ep-CAMhigh/CD49f + cells during sorting and in functional assays (to obtain enough cells) and compared them with the bulk of Ep-CAMhigh/CD49f + cells (CD44high expression levels were identified as cells with only 1 tick above 10 FI i.e. 2000 FI).

Importantly, we did not find any trend in Treg or Treg subset frequencies based on the time of sample collection post-transplantation. Due to the small numbers of Treg recovered from the samples we are not able to sort and perform functional assays to measure suppressive capacity of these CD3+CD4+CD25hiFoxp3+CD45RA CCR7+ cells.

And when you begin to look, you begin to see all sorts of adaptive and functional tendencies that can come out of that experiential response to aesthetics.

EST sequence processing and functional data sorting were performed using PipeOnline version 2.0 [ 79, 83].