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CD4+ Cdouble-positivetive (DP), CD4− Cdouble-negativetive (DN), CD4+ and CD8+ single-positive (SP) subpopulations of thymocytes were sorted by a FACS Vantage cell sorter as previously described [3].
Barrett's epithelial cell populations were purified from endoscopic biopsies and esophagectomy samples by means of Ki67/DNA content flow sorting of diploid G1, 4N, and aneuploid cell populations on a Coulter Elite ESP cell sorter, as described previously [20] [22].
Live cells were sorted with a FACSAria cell sorter as they excluded propidium iodide.
Mouse MMTV-Wnt1 breast tumors were digested and CD45-Thy1+CD24+ murine breast cancer cells were isolated using a cell sorter as described previously (Cho et al., 2008).
piRNA Sensor EMS Screen F2 or F3 offspring of mutagenized worms were sorted using a Copas Biosort Large-Particle Sorter as described in (Bagijn et al).. Worms sorted as 'GFP ON' were re-selected for sensor de-silencing using an FBS10 Fluorescence Microscope System (Kramer Scientific).
FACS analysis of hematopoietic tissues (spleen, bone marrow, thymus and lymph nodes) was carried out on an LSR1 (Becton Dickinson, Franklin Lakes, NJ, USA) machine, and cell sorting was performed using a MoFlo (Cytomation, Boulder, CO, USA) high-speed cell sorter as described.
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Formerly known as Shuffle Master, SHFL makes products like automatic card shufflers and roulette chip sorters, as well as proprietary table games and electronic gambling machines.
In the realm of cell separation for screening, there has been significant progress in miniaturized flow-based optical sorters as well as in sorting following static microscopic observation.
The percentage of annexin-Vlow (i.e. viable) or annexin-Vhigh (i.e. apoptotic) cells was determined using the data obtained by fluorescence-activated cell sorter analysis as described [36] [37].
CD34 positive cells were purified with magneted activated cell sorter (MACS) as already reported [ 21].
Apoptosis was determined by annexin V-FITC/PI staining/fluorescence-activated cell sorter analysis as described in Material and methods.
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