For gene expression studies, T helper cells were enriched from the allergen-challenged PBMC using anti-CD4-coated paramagnetic microbeads and a MACS (magnetic cell sorter) system according to the instructions of the manufacturer (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany).
DCs and CD8+ T cells were obtained from spleen cells of CBF1 mice using the magnetic cell sorter system MACS according to the manufacturer's protocol using anti-CD11c and anti-CD8 antibody-coated microbeads (Miltenyi Biotec), respectively.
CD11c+ spleen cells of naïve mice and CD4+ T and CD8+ T spleen cells of mice immunized with OVA-liposome conjugates were prepared with the magnetic cell sorter system MACS, according to the manufacturer's protocol using anti-CD11c, anti-CD4 and anti-CD8 antibody-coated microbeads (Miltenyi Biotec).
The proportion of GFP-positive cells was determined using MoFlo cell sorter (Beckman Coulter) according to the manufacturer's protocol.
The chromosomes obtained from the cell line of a male specimen (TK165824) were sorted on a MoFlo dual-laser cell sorter and isolated according to size and base-pair composition [ 16] at the Wellcome Trust Sanger Institute, UK.
Annexin V binding was assayed by fluorescence-activated cell sorter (FACS) analysis according to the ApoAlert Annexin V protocol (BD Biosciences Clontech Palo Alto, CA, USA) as previously described [ 17, 21, 23, 24].
Sca-1-labelled cells were sorted by FACS using a DakoCyomation MoFlo sorter into 3 populations according to the mean fluorescence intensity (MFI) of Sca-1 expression [2].
To this day it relies on a 1948 IBM 402 punchcard system for inventory and accounting along with an IBM 83 sorter and IBM 129 keypunch, according to the firm's long-time repairman, Duwayne Lafley, who lives in New Mexico.
The multiport consists of a reflections (R), spiral-phase-plate (SPP) that add a constant value of, a mode sorter that sorts OAM modes according to their parity (even/odd), a 50/50 beam splitter (BS) and a coherent mode projection (CMP).
The researchers created an assortment of "fluidic machines" with this system, which include self-assembling fluid lenses and a rainbow droplet sorter that can arrange droplets according to their concentration.
