Although, c-Myc is maximally induced between 2 and 4 hours following serum stimulation (Fig. 4), the 6 hour time point was chosen for sorting to ensure that cells had entered the cell cycle and because EFGP-Luc has a much longer half life (~15 hrs, data not shown) than the short, 20-30 minute half life of c-Myc (21, 23, 24).
Often there is a test of some sort to ensure that the worker is not taking a job that a native might fill usually a meaningless formality, but one of several that allow endless opportunities for bureaucratic delay.
It's putting your toddler to bed as he grips your hand or has you in a choke hold of sorts to ensure you won't escape.
Stable transfectants of these proteins were FACS-sorted to ensure that they expressed similar levels of the fluorescent reporter protein.
Both the full-length and open forms of Drosophila Dia, tagged with GFP, were stably expressed in MDCK cyst culture, which were subsequently FACS-sorted, to ensure selection of cysts displaying similar GFP expression levels.
Our analysis was guided by Grounded Theory techniques [ 32], whereby we read and re-read transcripts, coded, and identified categories/themes (and similarities and differences between these), sorted data to ensure that the concepts/theories were appropriate and noted differences between different groups of individuals [ 32, 33].
The main objective is to determine the location and the capacities of the sorting facilities to ensure compliance with the new regulation and prevent the wood from being massively landfilled.
They give creators more power to moderate and block comments, and add new sorting mechanisms to ensure better, more relevant discussions appear at the top.
The transduced cells were sorted twice using fluorescence-activated cell sorting (FACs) to ensure 100% of the cells stably expressed the H2B-GFP or H2B-mCherry protein.
To avoid bottlenecks, operators use techniques like "traffic shaping," which sorts traffic to ensure basic service for all, or "throttling," which applies a general brake on large streams of data.
