All offspring and parents are evaluated and sorted, and subsequently deleting the worst extra individuals.
To assign quality values to the ROC curves, the AUC values were sorted and subsequently allocated to three bins of sizes 5, 18, and 2. Finally the bins were assigned quality values of -1, 0, and 1, respectively.
The mosaic distribution of the two V1R2 allele groups across the East-African cichlids suggests that they emerged prior to the radiation of the LT species flock and were subsequently sorted into LT, LM, and LV (see later Discussion).
CD11c+MHC II+ cells were first sorted for bulk yield (Figure S1A), then single cells were subsequently sorted into 96 well plates based on CFSE positivity (Figure S1B).
Seaweeds were subsequently sorted out, washed thoroughly with water, shade dried and finally ground into fine powder.
Fetal E/e or e/e Rh genotype was determined correctly in 15 of 16 sample fractions that underwent magnetic bead separation with CD45 and were subsequently sorted into positive and negative fractions (94%).
CD3+CD4+ Th cells were subsequently sorted by flow cytometry.
CD4+-T cells were positively selected with magnetic beads and subsequently sorted with autoMACS (Miltenyi Biotec, Bergisch Gladbach, Germany).
To evaluate mitochondrial function, spermatozoa were stained with rhodamine123 (Rh123) and propidium iodide (PI) and subsequently sorted by flow cytometry.
Neuronal activity was recorded by the Digital Lynx system (Neuralynx, Tucson, AZ) and subsequently sorted individually by manual parameters with the Offline Sorter (Plexon, Dallas, TX).
