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The Giemsa staining of the sorted specimens revealed large cells with expanded cytoplasm containing vacuoles, suggesting that they are of the monocyte-macrophage lineage (Fig. 3b, right photo (iv)).
The Gr-1high cells clustered as a distinct population in the scatter analysis (FSC vs. SSC; Fig. 3b, top right), and showed the neutrophil morphology in the sorted specimens [Fig. 3b, Photo (i)].
Similar(58)
The time-based and rapid-blitz strategies were 54% and 50% similar, respectively, to the liberal re-sort method, which is made up of re-sorted specimens from those two strategies.
However, DNA analysis of non flow-sorted specimens underestimates the number and longitudinal complexity of breaks detected by array CGH.
Despite this wide range of cellularity, skewed toward the lower end of the spectrum, Figure 4a and b show that only a weak correlation exists between the RNA integrity number, GEP70 score and pre-sorted specimen percentage of CD138+ cells (r = 0.13 and 0.010, respectively).
There was strong desire to receive more clinically relevant antibiograms based on outpatient cultures and sorted by specimen type (e.g. abscess, urine etc).
In summary, we used a novel approach to analyze the data obtained with flow-sorted tumor specimens using array CGH, which provides a new insight into the genomic complexity of breast cancer.
When CD66cneg and positive fraction was FACS-sorted of heterogeneous specimens (lymphoblasts partly positive for CD66c) the level of CEACAM6 was observed higher in CD66cneg cells and lower in CD66cpos cells as compared to uniform populations.
All specimens were sorted and identified to the species level.
Datasets previously obtained from five non-sorted whole-tissue tumor specimens and their matched non-cancer specimens were used for comparison [ 14].
Establishing the extent of agreement between semi-quantitative immunostaining data and gene array data obtained from sorted cell populations and tissue specimens is important to account for possible discrepancies between these two very different methods.
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