ESCs were transfected as in the silencing assay with pCAG-eGFP-IB and pCMV-mRFP, and GFP-positive cells were sequentially sorted with a FACSVantage or FACSAria II (Becton Dickinson, Heidelberg, Germany) at days 2, 6 and 10 after transfection.
Randomly sorted numbers were called sequentially.
Adjacent loci were clustered by sequentially sorting genotypes of multiple plants, and clusters of loci with similar segregation patterns were manually joined by moving the rows to minimize the total number of recombination events observed.
The list of all potential controls was then sorted randomly by case number and sequentially examined via chart review until 2 controls were identified for each case.
After the washout period, animals of the same age and gender were sorted by body weight and assigned sequentially to the adjuvant arthritis or control groups (Table 1).
He later sorted out some 90 slides and projected them sequentially as a piece titled "Slides of a Changing Painting," which is on view near the beginning of the Hirshhorn show.
The scaffolds aligning to each sorghum chromosome were further sorted into A and B pseudo-chromosomes, by sequentially placing them into two groups based on overlap.
The above steps are continued till all exons have been sorted as distinct and variant, after which they are sequentially numbered.
After determining the orthologous sequences, we sorted them in decreasing order and added orthologous sequences from other species sequentially to find the most common set.
