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Samples were sorted on the DEPArray in RPMI-1640 medium.
Samples were sorted on the DEPArray in RPMI-1640 and isolations of 1 or 2 single tumour cells, groups of 3 to 10 tumour cells and a group of 10 WBCs were performed.
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Cells were sorted on the Aria Cell Sorter (BD Biosciences).
In cell sorting experiments, GBM cells were analysed and then sorted on the basis of CD133 expression.
Cells were sorted on FACS Aria (BD) at the Stanford Shared FACS Facility.
Table sorted on% congruence.
Tumour cells visualised on the DEPArray were defined using standard CellSearch CTC criteria as described elsewhere (Riethdorf et al, 2007).
Therefore, we have tried to estimate the extent of cell loss that has to be taken into account when processing CellSearch samples on the DEPArray.
The percentage of cells enumerated on the CellSearch system that could be re-identified on the DEPArray system was used as a measure to estimate the total extent of cell loss.
After being processed on the CellSearch System, CellSearch cartridges were stored at 4 °C for a mean duration of 5 days (maximum 15 days) before analysis on the DEPArray system.
After enrichment and enumeration of both cell populations on the CellSearch system, CellSearch cartridges were removed from their Magnest (Veridex LLC) and stored protected from light at 4 °C until enumeration of the same sample was performed on the DEPArray.
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