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Every 17 000÷5500≈3rd participant was selected from a sorted name list of 17 000 eligible nurses, with the first participant chosen at random.
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Orphaned reads (mate of reads whose one end mapped to transgene) from name sorted sequence alignment map (SAM) [ 23] file were then extracted using custom Perl scripts.
To assemble a sequence matrix for phylogenetic inference, each protein cluster was aligned using ClustalW (Larkin et al., 2007) (default parameters), trimmed for high-quality alignment blocks with GBlocks (Castresana, 2000) (default settings), sorted by species name, and concatenated.
Recorded units were spike-sorted (Figure 1).
MCF7 cells were infected with the lentivirus containing either CT45A1 cDNA or GFP cDNA, and the GFP-positive cells were sorted using flow cytometry, and the sorted MCF7 cells were named as MCF7-CT45A1 and MCF7-V, respectively.
This was sorted based on last name and split into two groups (personalised email versus generic email).
All individual medication names were sorted using existing medication sub-classes classification from the ODB database, after which medication sub-classes were grouped into specific medication classes.
We used a publicly available list of 6,534 tissue-specific gene names (under Tissue-Specific Genes based on Expressed Sequence Tags (ESTs)) from the TiGER database (gene names were sorted and redundancy was removed - Additional file 10) and we searched for their promoters in the Eukaryotic Promoter Database in which we found 2,369 promoters.
Patients' names were randomly sorted and patients contacted by telephone until approximately 15% of patients from each practice agreed to participate in the field survey.
Patient names were randomly sorted and patients contacted until a sample of approximately 15% of the patients from each practice agreed to an interview.
Patient names were randomly sorted and patients contacted by telephone until a sample of approximately 15% of the patients from each practice agreed to an interview.
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