In N2a neuronal cells, transient LB1 overexpression increased nuclear stiffness of isolated, FACS-sorted nuclei (mean stiffness of LB1/EGFP, 2.28±0.60 mN/m; EGFP alone, 1.19±0.30 mN/m; P<0.01, Mann-Whitney rank sum test) and in living adherent cells (Fig. 5 B ).
It may even be said that perfect sorting is more important for the latter, since more neatly sorted recyclable waste means better recyclability and higher profitability for recyclers.
(F ) Quantitative RT-PCR analysis of Wdr47 mRNA in sorted DP thymocytes (mean ± s.d., n = 2 3).
Rows may additionally be sorted alphabetically, by mean abundance or by coefficient of variance.
(B ) Quantitative RT-PCR analysis of Mrps5 and Rabggtb mRNA in sorted DP thymocytes (mean ± s.d., n = 3).
Descriptive statistics on the average cDNA pool coverage obtained for each enzyme combination across all 92 species (see additional file 1), sorted by decreasing mean coverage.
Each pool of sorted fluorescent neurons (mean ± sd: 102 ± 40.5 neurons, range: 44 214 neurons) from a single brain was used for a separate sample from which RNA was extracted and then reverse transcribed, amplified, sequenced, and analyzed.
Moreover, males in the second study were size sorted (reducing the mean maximum size difference within each tank from 40 mm in study 1 to 12 mm in study 2) in order to reduce the potential confounding effect of size on reproductive performance.
When the mean consecutive difference/mean sorted difference (MCD/MSD) value exceeded 1.25, which means the inter-potential interval was influenced by the variations in the firing rate, we used the MSD instead of the MCD to represent the neuromuscular jitter, because the MSD is a mathematical algorithm that reduces the effect of this factor (Sanders and Stålberg 1996).
In these plots, residuals were sorted, and the sorted values were plotted against the expected sorted birth weight values from a normal distribution with the same mean and variance.
